Anti-CD337/NKp30 Monoclonal Antibody for IF/ICC, FC - O14931

Core Product Specifications and Parameters

Biological Background: CD337/NKp30 Function and Localization

• CD337/NKp30, also known as natural cytotoxicity triggering receptor 3 (NCR3), activating natural killer receptor p30, or NKp30, is a cell membrane receptor encoded by the NCR3 gene.
• It is selectively expressed on the surface of resting and activated natural killer (NK) cells, with weak expression observed in the spleen.
• The receptor plays a critical role in NK cell-mediated cytotoxicity by recognizing extracellular ligands such as BAG6 and NCR3LG1.
• Upon ligand engagement, NKp30 triggers NK cell activation, leading to targeted killing of tumor cells and other abnormal cells.
• Beyond direct cytotoxicity, NKp30 engagement promotes myeloid dendritic cell (DC) maturation by eliminating immature DCs and inducing NK cells to secrete TNF-α and IFN-γ.
• Structurally, NKp30 is a transmembrane glycoprotein containing a disulfide bond and an immunoglobulin-like domain, with a molecular weight of approximately 21.6 kDa.
• The protein undergoes alternative splicing, yielding isoforms with distinct biological properties, and its 3D structure has been elucidated.

Experimental Guidance and Technical Tips

• For immunofluorescence, include proper fixation and permeabilization steps appropriate for cell surface receptors; validate in sample systems where NKp30 expression is expected.
• For flow cytometry (intracellular staining protocol), ensure cells are fixed and permeabilized prior to staining; confirm positive signals in NK cell lines or primary human NK cells.
• Consider using an isotype control and comparing unstimulated versus stimulated NK cells to assess staining specificity.
• The antibody is validated for human reactivity; cross-reactivity with other species has not been tested.
• Note that this antibody is conjugated to ; verify fluorophore compatibility with your detection system and panel design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, including high-quality monoclonal antibodies like this anti-NKp30 reagent. Whether you require validated antibody pairs, optimized antibodies, or ancillary reagents, our one-stop platform supports your workflow from early discovery through clinical application.