Anti-CD335/NKp46 Rabbit Polyclonal Antibody for WB and ELISA - Q8C567

Core Product Specifications and Parameters

Biological Background: CD335 Function and Localization

• CD335, also known as NKp46, Ly94, or Ncr1, is a natural cytotoxicity triggering receptor (NCTR) encoded by the Ncr1 gene in mice.
• It functions as a cytotoxicity-activating receptor that enhances the efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
• CD335 is selectively expressed on the surface of NK cells, acting as a key marker and functional receptor for innate immune surveillance.
• The protein is a transmembrane receptor with an extracellular region containing immunoglobulin-like domains, a single-pass transmembrane helix, and a short cytoplasmic tail.
• Post-translational modifications include glycosylation and disulfide bond formation, likely important for folding and stability.
• As a member of the immunoglobulin superfamily, it plays a role in recognizing ligands on target cells to trigger NK cell cytotoxicity.

Experimental Guidance and Technical Tips

• Given that the immunogen spans the extracellular domain (aa 17-255), the antibody may be suitable for detecting native CD335 in applications targeting surface epitopes; however, additional validation is necessary for techniques such as flow cytometry or immunohistochemistry.
• For Western blotting, a predominant band around 37 kDa is expected, though glycosylation may cause a slight shift; use lysates from NK cell lines (e.g., YAC-1) or enriched NK cell populations as positive controls.
• In ELISA, titrate the antibody against recombinant CD335 or cell lysates to optimize the detection range; include a blocking step to reduce nonspecific binding.
• Since the antibody is polyclonal, batch-to-batch variability may occur; consider validating each new lot in your target application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop procurement platform for IVD raw materials, supporting researchers and manufacturers from concept to clinical application. This anti-CD335 rabbit polyclonal antibody is tailored for NK cell research and immunoassay development, complementing our catalog of validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare target sourcing or customized solutions, our team provides expert technical consulting to accelerate your project.