Anti-CD335/NKp46 Monoclonal Antibody for IF/ICC, FC, ELISA - O76036

Core Product Specifications and Parameters

Biological Background: CD335/NKp46 Function and Localization

• CD335/NKp46, also designated natural cytotoxicity triggering receptor 1 (NCR1) or lymphocyte antigen 94 homolog (LY94), is a cell surface receptor primarily expressed on natural killer (NK) cells.
• It functions as a cytotoxicity-activating receptor that enhances activated NK cell–mediated lysis of tumor cells.
• The receptor is selectively expressed on both resting and activated NK cells, highlighting its role in innate immune surveillance.
• CD335/NKp46 is localized to the cell membrane and features two extracellular immunoglobulin-like domains, a transmembrane helix, and a short cytoplasmic tail.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are important for structural stability and ligand interaction.
• Multiple protein isoforms are generated through alternative splicing, with observed molecular weights ranging from ~21 kDa to ~34 kDa, depending on glycosylation and isoform.
• Structurally, CD335/NKp46 belongs to the immunoglobulin superfamily and is recognized as a key NK cell marker in flow cytometry and immunofluorescence studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 22–254), making this antibody suitable for detecting surface-expressed CD335/NKp46 in live cells without permeabilization.
• For flow cytometry (FC), consider using a directly conjugated format or an appropriate secondary antibody; validate staining on NK cell lines (e.g., NK-92) or primary NK cells with an isotype control.
• In immunofluorescence/immunocytochemistry (IF/ICC), fixation with paraformaldehyde is recommended, and no permeabilization is required for membrane staining; co-stain with markers like CD56 to confirm NK cell identity.
• For ELISA applications, ensure that the recombinant target protein carries the extracellular domain and that the assay buffer conditions are optimized for antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for sourcing high-quality IVD raw materials, including validated monoclonal antibodies like this anti-CD335/NKp46 clone for immune-oncology and NK cell research. We support diagnostic manufacturers and laboratories with bulk antibody supply, custom development, and ancillary reagent procurement, streamlining your path from concept to clinic. Reach out to our team for access to validated antibody pairs, rare-target reagents, and scalable production solutions tailored to your diagnostic needs.