Anti-CD33 Rabbit Polyclonal Antibody for WB, ELISA - P20138

Core Product Specifications and Parameters

Biological Background: CD33 Function and Localization

• CD33, also known as Siglec-3 or gp67, is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family, characterized by an N-terminal V-set immunoglobulin domain that mediates sialic acid recognition.
• It functions as an inhibitory receptor on myeloid cells, maintaining immune cells in a resting state through ITIM-mediated signaling (PubMed:10611343, PubMed:11320212, PubMed:15597323). Related references: PMID:10611343 PMID:11320212 PMID:15597323
• CD33 preferentially binds alpha-2,3- and alpha-2,6-linked sialic acids, with higher avidity for the latter (PubMed:7718872). Related references: PMID:7718872
• Upon ligand engagement, its cytoplasmic ITIMs are phosphorylated by Src-like kinases, recruiting phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 to downregulate signaling (PubMed:10206955, PubMed:10556798, PubMed:10887109, PubMed:28325905). Related references: PMID:10206955 PMID:10556798 PMID:10887109 PMID:28325905
• CD33 localizes to the cell membrane and peroxisomes, consistent with its roles in cell adhesion and signaling.
• It is expressed specifically on monocytic/myeloid lineage cells and, in the brain, primarily on microglia.
• The protein undergoes alternative splicing, yielding isoforms with potentially distinct functions.
• Post-translational modifications include phosphorylation on ITIM tyrosines and N-linked glycosylation, contributing to its regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen was designed against the cytoplasmic region (aa 265-364), which is conserved between isoforms; this may allow detection of total CD33.
• For Western blotting, consider an initial dilution range of 1:500 to 1:2000 and validate with appropriate positive (e.g., human monocyte lysate) and negative controls.
• For ELISA, the antibody can be employed as a capture or detection reagent; assess cross-reactivity with other Siglecs if multiplexing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. This anti-CD33 antibody supports research in myeloid cell biology, immunology, and neuroinflammation. Contact us to discuss how we can streamline your diagnostic development from concept to clinic.