Anti-CD33 Rabbit Monoclonal Antibody for WB, ELISA - P20138

Core Product Specifications and Parameters

Biological Background: CD33 Function and Localization

• Myeloid cell surface antigen CD33 (also known as Siglec-3, p67, gp67) is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family.
• CD33 mediates cell-cell interactions and maintains immune cells in a resting state. Related references: PMID:10611343 PMID:11320212 PMID:15597323
• Preferentially recognizes and binds alpha-2,3- and alpha-2,6-linked sialic acid-bearing glycans. Related references: PMID:7718872
• Upon ligand engagement, ITIM motifs are phosphorylated by Src-like kinases (e.g., LCK), recruiting SHP-1 and SHP-2 phosphatases that dephosphorylate downstream signaling molecules. Related references: PMID:10887109 PMID:28325905 PMID:10206955 PMID:10556798
• The repressive effect on monocyte activation requires phosphoinositide 3-kinase/PI3K. Related references: PMID:15597323
• Localizes to the cell membrane and peroxisomes.
• Expressed in monocytic/myeloid lineage cells; in the brain, primarily on microglial cells.
• The CD33 gene undergoes alternative splicing, producing multiple isoforms; the protein is glycosylated, phosphorylated, and contains disulfide bonds.

Experimental Guidance and Technical Tips

• For Western blot, consider a detection band around 40 kDa under reducing conditions, consistent with the predicted molecular weight of CD33.
• For ELISA, the antibody may be used for detection of recombinant or native CD33; appropriate positive controls such as myeloid cell lysates are recommended.
• The immunogen encompasses the extracellular domain (amino acids 18-259), suggesting the antibody likely recognizes the extracellular portion of CD33, suitable for detection of surface-expressed antigen.
• Since this is a monoclonal antibody, lot-to-lot consistency is expected; validate in your specific sample system to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with one-stop access to high-quality IVD antibodies and related raw materials. For CD33-targeted research, we offer validated monoclonal antibodies (such as this CD33 Rabbit mAb) optimized for applications like WB and ELISA, along with bulk ancillary reagents and customized sourcing for rare targets from concept to clinical use. Our integrated supply chain ensures reliable, consistent materials for your assay development needs.