Anti-CD32A + CD32B + CD32C Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P12318 / P31994 / P31995

Core Product Specifications and Parameters

Biological Background: CD32A + CD32B + CD32C Function and Localization

• CD32A (FCGR2A) is a low-affinity immunoglobulin gamma Fc region receptor that binds the Fc portion of IgG, mediating cellular responses against pathogens and soluble antigens.
• The antibody targets CD32A, CD32B, and CD32C, which are distinct but highly homologous receptors encoded by the FCGR2A, FCGR2B, and FCGR2C genes, respectively. The immunogen corresponds to a conserved N-terminal extracellular region.
• CD32A promotes phagocytosis of opsonized antigens, playing a critical role in immune complex clearance and antimicrobial defense.
• The receptor is a transmembrane protein localized primarily at the cell membrane, as indicated by subcellular location data.
• Expression is found on monocytes, neutrophils, and eosinophil platelets, consistent with its role in innate immune effector cells.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, which may influence receptor stability and signaling.
• Alternative splicing of the FCGR2A gene generates multiple isoforms, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein spanning amino acids 1–100 of human CD32A/CD32B/CD32C, a region predicted to be extracellular. This design may facilitate detection of both unprocessed and membrane-bound forms under various denaturing conditions.
• For Western blotting, expect a band near 35 kDa (CD32A) or 34 kDa (CD32B). Optimize sample preparation (e.g., reducing vs. non-reducing conditions) and antibody dilution to resolve potential isoform overlaps.
• In IHC-P, validate staining in appropriate positive control tissues, such as lymphoid organs rich in myeloid cells. Antigen retrieval protocols may need optimization for optimal signal.
• For ELISA, pairing with a detection antibody targeting a non-overlapping epitope could improve sensitivity. Empirical titration and specificity testing in the relevant sample matrix are recommended.

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