Anti-CD328 Polyclonal Antibody for WB, ELISA - Q9Y286

Core Product Specifications and Parameters

Biological Background: CD328 Function and Localization

• Sialic acid-binding Ig-like lectin 7 (Siglec-7), also known as CD328, is a putative adhesion molecule that preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acids and disialogangliosides. The sialic acid recognition site may be masked by cis interactions on the same cell surface.
• Functions as an inhibitory receptor in immune responses: upon ligand-induced tyrosine phosphorylation, it recruits cytoplasmic phosphatases via SH2 domains, blocking signal transduction through dephosphorylation of signaling molecules.
• Mediates inhibition of natural killer (NK) cell cytotoxicity, playing a key role in regulating innate immunity.
• May also be involved in hematopoiesis; in vitro studies show it inhibits differentiation of CD34+ cell precursors toward the myelomonocytic lineage and suppresses proliferation of leukemic myeloid cells.
• Expressed predominantly on resting and activated NK cells, and at lower levels on granulocytes and monocytes. High expression is found in placenta, liver, lung, spleen, and peripheral blood leukocytes.
• Localized to the cell membrane as a single-pass type I membrane protein.
• Contains immunoglobulin-like domains, disulfide bonds, and glycosylation sites; classified under the immunoglobulin superfamily and lectin family.
• Alternative splicing produces multiple isoforms (as indicated by the keyword "Alternative splicing").

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (aa 18-144) of human CD328, which may favor recognition of the native protein in applications such as flow cytometry or ELISA; however, validation in the specific experimental system is advised.
• For Western blotting, a band around 51 kDa is expected in human NK cell lysates (e.g., NK-92 cell line) or tissues like spleen and lung.
• Since this is a polyclonal antibody, lot-to-lot consistency should be verified for quantitative assays.
• In ELISA, pairing with a monoclonal anti-CD328 antibody may improve specificity; consider testing with recombinant CD328 protein as a positive control.

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CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including this anti-CD328 polyclonal antibody for NK cell and cancer immunotherapy studies. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing solutions for rare targets. Partner with us to streamline your development from concept to clinic.