Anti-CD326/EpCAM Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q99JW5

Core Product Specifications and Parameters

Biological Background: CD326/EpCAM Function and Localization

• CD326/EpCAM is a type I transmembrane glycoprotein also known as epithelial glycoprotein 314 (EGP314), protein 289A, and tumor-associated calcium signal transducer 1 (Trop-1).
• Functions as a homophilic cell adhesion molecule mediating interactions between intestinal epithelial cells and intraepithelial lymphocytes, contributing to mucosal immune barrier integrity.
• Plays a role in embryonic stem cell proliferation and differentiation; up-regulates expression of FABP5, MYC, and cyclins A and E (by similarity).
• Localizes to the lateral cell membrane and tight junctions, consistent with its role in cell-cell adhesion.
• Contains disulfide bonds and is glycosylated, suggesting structural stability and surface exposure.
• Features a signal peptide, an extracellular domain with repeats, a single transmembrane helix, and a short cytoplasmic tail.
• Belongs to the EpCAM/TACSTD family of cell adhesion molecules implicated in epithelial tissue organization and tumorigenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 24-266), ensuring recognition of the native surface protein in applications like IF and IHC.
• For Western blotting, anticipate a band around 35 kDa (predicted molecular weight); post-translational modifications such as glycosylation may cause the observed band to shift slightly higher.
• In immunofluorescence and immunohistochemistry, optimize fixation and permeabilization steps for membrane antigens to preserve epitope accessibility.
• Cross-reactivity with human and rat EpCAM indicates a conserved epitope; validate signal specificity using appropriate positive controls from each species.
• As a monoclonal antibody, lot-to-lot consistency supports reliable performance in ELISA and other plate-based assays; consider pairing with a suitable detection antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for navigating the IVD raw material landscape, providing seamless access to validated antibody pairs, optimized monoclonals, polyclonals, and bulk ancillary reagents. Whether your research focuses on epithelial adhesion, tumor biomarkers, or mucosal immunity, CamelBio streamlines your procurement of rare-target materials and consistent lots—supporting your assay development from concept to clinic.