Anti-CD324/E-Cadherin Rabbit PolymAb® for WB, IF-P, IHC-P, ELISA - P09803

Core Product Specifications and Parameters

Biological Background: CD324/E-Cadherin Function and Localization

• Cadherin-1 (CD324/E-Cadherin), also known as uvomorulin (Um), ARC-1, E-cad, and L-CAM, is a classical cadherin from the cadherin superfamily.
• It mediates calcium-dependent homophilic cell-cell adhesion, playing essential roles in cell sorting, epithelial cell mobility, and proliferation (PubMed:11976333).
• Related references: PMID:11976333
• Through interaction with AMOTL2, it promotes organization of radial actin fibers and cellular response to contractile forces, linking cell junctions to the cytoskeleton (By similarity).
• Participates in early desmosome assembly by facilitating recruitment of DSG2 and DSP to desmosome plaques; also acts as a ligand for integrin alpha-E/beta-7 (By similarity).
• Possesses tumor suppressor activity and promotes non-amyloidogenic degradation of Abeta precursors via its cytoplasmic fragment E-Cad/CTF2 (By similarity).
• Subcellularly localizes to adherens junctions, desmosomes, cell membrane, endosomes, trans-Golgi network, and cytoplasm, reflecting its dynamic trafficking.
• Expressed in non-neural epithelial tissues, including skin keratinocytes, organ of Corti pillar cells, and granuloma macrophages (PubMed:30639848, PubMed:27760340, PubMed:18079750, PubMed:27375112, PubMed:30660666).
• Related references: PMID:30639848, PMID:27760340, PMID:18079750, PMID:27375112, PMID:30660666

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 157–369 within the extracellular cadherin repeat domains, suggesting suitability for detecting both full-length and processed forms of cadherin-1 in denaturing and native applications.
• In WB, the predicted molecular weight is 98kDa; use appropriate gel acrylamide percentages (e.g., 8–10%) and optimize transfer conditions for high-molecular-weight proteins.
• For IHC and IF, antigen retrieval and permeabilization should be optimized; consider validating in mouse/rat positive tissues such as epithelial linings or cell lines.
• Include relevant controls (e.g., knockout/knockdown lysates, siRNA-treated cells) to confirm specificity, especially given the high homology among cadherins.
• In ELISA, use recombinant cadherin-1 protein or cell lysates as positive controls and include buffer-only blanks to monitor background.

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