Anti-CD31/PECAM1 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P - P16284

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• Essential cell adhesion molecule for leukocyte transendothelial migration (TEM); Tyr-690 phosphorylation is required for LBRC trafficking and TEM efficiency. Related references: PMID:17580308 PMID:19342684
• Mediates endothelial cell-cell adhesion through trans-homophilic binding and facilitates neutrophil TEM via heterophilic interaction with CD177. Related references: PMID:27958302 PMID:17580308
• Regulates phagocytosis: homophilic ligation prevents viable leukocyte clearance, while apoptotic leukocyte tethering promotes phagocytosis. Related references: PMID:12110892
• Modulates bradykinin receptor BDKRB2 and ERK1/2 signaling; induces susceptibility to atherosclerosis (by similarity). Related references: PMID:18672896
• Does not protect against apoptosis.
• Expressed on platelets, leukocytes, and concentrated at endothelial cell junctions; multiple isoforms show tissue-restricted patterns (e.g., Long form predominant, Delta12 in trachea, Delta14-15 in lung).
• Subcellularly located at cell membrane, membrane rafts, and cell junctions; characterized by immunoglobulin-like domains, disulfide bonds, glycosylation, palmitoylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal cytoplasmic region (amino acids 630–738) of human CD31/PECAM1, a domain conserved in mouse, supporting the reported cross-reactivity.
• For Western blotting, the predicted molecular weight is ~83 kDa; note that the mature glycosylated form may migrate at ~130 kDa in some cell types.
• For immunofluorescence on paraffin-embedded sections (IF-P) and immunohistochemistry (IHC-P), optimal antigen retrieval conditions should be determined empirically.
• Validate the antibody in the relevant sample system (e.g., HUVECs or leukocyte cell lines) due to tissue-specific isoform expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories in acquiring high-quality antibodies, antigens, and ancillary reagents. For CD31/PECAM1-related studies in vascular biology, inflammation, and cell adhesion, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to streamline your assay development and production. Our global network enables reliable supply of rare-target raw materials, ensuring continuity from concept to clinic.