Anti-CD31/PECAM1 Rabbit Monoclonal Antibody for WB, IF, IHC, ELISA - P16284

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• CD31/PECAM1 is a cell adhesion molecule essential for leukocyte transendothelial migration (TEM) under inflammatory conditions; phosphorylation at Tyr-690 is critical for this process. Related references: PMID:17580308, PMID:19342684
• It mediates homophilic interactions at endothelial cell junctions, contributing to vascular integrity. Related references: PMID:27958302
• PECAM1 regulates phagocytosis: it delivers a detachment signal to prevent phagocytosis of viable leukocytes while tethering and promoting clearance of apoptotic cells. Related references: PMID:12110892
• It modulates bradykinin receptor BDKRB2 activation and downstream ERK1/2 signaling in endothelial cells. Related references: PMID:18672896
• PECAM1 is expressed on platelets, leukocytes, and at endothelial cell borders. Multiple isoforms exist: Long form predominates; Delta12 in trachea; Delta14-15 in lung; Delta14 in heart; Delta15 in brain, testis, ovary, etc. Related references: PMID:18388311, PMID:21464369, PMID:12433657
• The protein contains immunoglobulin domains, disulfide bonds, and undergoes post-translational modifications such as glycosylation, palmitoylation, and phosphorylation.
• Subcellular localization includes the cell membrane, membrane rafts, and cell junctions.

Experimental Guidance and Technical Tips

• The immunogen covers the cytoplasmic tail (aa 630–738); the antibody may detect both full-length and cleaved fragments under certain conditions.
• For Western blot, a band at approximately 83 kDa is expected in human, mouse, or rat endothelial cell lysates; validate in the relevant sample system.
• In immunofluorescence and immunohistochemistry, membrane and junctional staining patterns are anticipated; optimize fixation and permeabilization protocols for your experimental setup.
• Consider using this antibody for ELISA applications; confirm appropriate coating and detection conditions in your assay.

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CamelBio provides diagnostic manufacturers and researchers with a streamlined procurement channel for high-quality IVD raw materials. From optimized monoclonal antibodies like this anti-CD31/PECAM1 clone to ancillary reagents and rare-target sourcing, our global network supports your cardiovascular and immunology assay development with consistent supply and technical consultation.