Anti-CD31/PECAM1 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q08481

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• PECAM1 (CD31) is a 130 kDa glycoprotein belonging to the immunoglobulin superfamily, serving as a major cell adhesion molecule on endothelial cells, platelets, and subsets of leukocytes.
• It mediates both homophilic (PECAM1-PECAM1) and heterophilic (e.g., with CD177) interactions, critically required for leukocyte transendothelial migration under inflammatory conditions.
• Tyr-679 phosphorylation in the cytoplasmic tail regulates trafficking to the lateral border recycling compartment, essential for directed migration of leukocytes across endothelial junctions.
• Homophilic ligation provides a detachment signal that prevents phagocytosis of viable leukocytes, while tethering apoptotic leukocytes to facilitate their clearance.
• PECAM1 modulates bradykinin receptor BDKRB2 activation and osmotic shock-induced ERK1/2 signaling in endothelial cells, linking adhesion to intracellular pathways.
• It contributes to susceptibility to atherosclerosis, as shown in mouse models. Related references: PMID:19048083
• Localized at cell membranes, lipid rafts, and cell junctions, consistent with its roles in adhesion and signaling.
• Post-translational modifications include disulfide bonds, glycosylation, palmitoylation, and phosphorylation; alternative splicing generates isoforms with distinct functional properties.

Experimental Guidance and Technical Tips

• The immunogen spans the intracellular C-terminal domain (aa 630–738); therefore, the antibody detects full-length membrane-bound PECAM1 and may not recognize soluble isoforms lacking the cytoplasmic tail. Validate specificity with appropriate negative controls.
• For western blotting, the predicted molecular weight is approximately 81 kDa, but extensive N‑linked glycosylation can cause migration at 120–130 kDa. Use endothelial cell lysates (e.g., HUVEC, bEnd.3) or platelet extracts as positive controls.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), PECAM1 exhibits membranous and junctional staining. For IHC-P, antigen retrieval may be required; test citrate or EDTA buffer and compare with isotype controls.
• For ELISA applications, consider coating recombinant PECAM1 extracellular domain or cell lysates, and optimize antibody dilution and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop solution for diagnostic manufacturers, laboratories, and research institutes, bridging raw-material sourcing, technical services, and consulting from concept to clinic. Our CD31/PECAM1 polyclonal antibody is part of an extensive portfolio of validated antibodies, antibody pairs, and ancillary reagents for IVD development in vascular biology, inflammation, and oncology. For bulk orders, rare targets, or custom sourcing, our expert team streamlines procurement to accelerate your project timelines.