Anti-CD31/PECAM1 Mouse Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P16284

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• CD31/PECAM1 is a cell adhesion molecule essential for leukocyte transendothelial migration (TEM) under most inflammatory conditions. It cycles through the lateral border recycling compartment (LBRC) during TEM, with phosphorylation of Tyr-690 playing a critical role in this process. Related references: PMID:17580308, PMID:19342684
• Trans-homophilic interactions of CD31/PECAM1 contribute to endothelial cell-cell adhesion at cell junctions. Related references: PMID:27958302
• Heterophilic binding to CD177 facilitates neutrophil transendothelial migration. Related references: PMID:17580308
• Homophilic ligation of CD31/PECAM1 transmits a detachment signal that prevents macrophage phagocytosis of viable leukocytes, while promoting the tethering and clearance of apoptotic leukocytes. Related references: PMID:12110892
• CD31/PECAM1 modulates bradykinin receptor BDKRB2 activation and regulates bradykinin- or hyperosmotic shock-induced ERK1/2 signaling in endothelial cells. Related references: PMID:18672896
• Multiple isoforms are generated by alternative splicing, with the long isoform predominating in most tissues. Isoform Delta12 is restricted to trachea, Delta14-15 to lung, and Delta14 and Delta15 show broad expression profiles including high levels in heart and platelets. Related references: PMID:12433657, PMID:18388311
• CD31/PECAM1 localizes to cell membranes, membrane rafts, and cell junctions. It is particularly concentrated at endothelial cell borders, consistent with its role in cell-cell adhesion and leukocyte diapedesis. Related references: PMID:18388311, PMID:21464369
• The protein is heavily glycosylated and contains immunoglobulin-like domains, disulfide bonds, and palmitoylation sites, contributing to its stability and membrane association.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 630-738 in the cytoplasmic tail, suggesting the antibody recognizes the C-terminal region. This may be useful for detecting total CD31/PECAM1 regardless of glycosylation or extracellular domain variants.
• For Western blotting, expect a band at approximately 83 kDa. Positive control: HUVEC or platelet lysates. Multiple bands or a band shift may occur due to post-translational modifications or isoforms; validate in your specific sample system.
• For immunofluorescence (IF-P), membrane and cell junction staining is anticipated, particularly at endothelial cell borders. Permeabilization may be required to access the intracellular epitope.
• For immunohistochemistry (IHC-P), membrane staining in vascular endothelial cells is expected. Optimize antigen retrieval and antibody dilution for your tissue type.
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with another clone targeting a different epitope may enhance sensitivity.
• Cross-reactivity with mouse allows dual-species studies. However, validate performance in each species' sample type independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner for diagnostic manufacturers and research laboratories worldwide. This anti-CD31/PECAM1 monoclonal antibody supports vascular biology, inflammation, and atherosclerosis research, and CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with rare target raw-material sourcing to accelerate your assay development from concept to clinic.