Anti-CD31/PECAM1 Monoclonal Antibody (Biotin) for FC - Q08481

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• CD31, also known as PECAM1, is a cell adhesion molecule of the immunoglobulin superfamily, encoded by the Pecam1 gene in mice.
• It plays a critical role in leukocyte transendothelial migration (TEM) under inflammatory conditions, with Tyr-679 essential for trafficking to the lateral border recycling compartment (LBRC) (By similarity).
• Homophilic interactions between endothelial cells contribute to cell-cell adhesion at junctions, while heterophilic binding with CD177 facilitates neutrophil transmigration (By similarity).
• CD31 regulates phagocytosis: homophilic ligation prevents macrophage ingestion of viable leukocytes, whereas it promotes tethering and phagocytosis of apoptotic cells (By similarity).
• It modulates bradykinin receptor BDKRB2 activity and downstream ERK1/2 signaling in endothelial cells (By similarity).
• CD31 is associated with susceptibility to atherosclerosis, as demonstrated in mouse models (PubMed:19048083). Related references: PMID:19048083
• Localized predominantly at the cell membrane, within membrane rafts, and at cell junctions (By similarity).
• Expressed in lung tissue and platelets, with multiple isoforms due to alternative splicing.

Experimental Guidance and Technical Tips

• The biotin conjugate enables direct detection in flow cytometry without a secondary antibody; titrate the antibody to determine the optimal working concentration for your cell type.
• The immunogen covers the extracellular domain (amino acids 18-220), which contains Ig-like domains involved in homophilic interactions; consider that epitope accessibility may be influenced by cell adhesion states.
• Validate cross-reactivity in your specific rat model, as reactivity with rat CD31 is reported but performance may vary with experimental conditions.
• For multicolor flow cytometry, pair with antibodies conjugated to spectrally distinct fluorophores and include appropriate isotype controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the supply of IVD raw materials for diagnostic manufacturers and research laboratories, offering validated antibody pairs, bulk ancillary reagents, and tailored sourcing for rare targets. For CD31/PECAM1 studies, we provide high-quality monoclonal antibodies to support endothelial biology, inflammation, and platelet research. Our integrated procurement process helps bridge the gap from discovery to clinical application with reliable, one-stop access.