Anti-CD31/PECAM1 Monoclonal Antibody (APC/Cyanine7) for Flow Cytometry - Q08481

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• Platelet endothelial cell adhesion molecule (PECAM1/CD31) is a transmembrane glycoprotein and member of the immunoglobulin superfamily, expressed as multiple isoforms (e.g., ~69, 77, 80, and 81 kDa) due to alternative splicing.
• PECAM1 plays a critical role in leukocyte transendothelial migration (TEM) under inflammatory conditions; Tyr-679 is essential for trafficking to the lateral border recycling compartment and efficient TEM.
• The protein mediates both homophilic (PECAM1-PECAM1) and heterophilic (e.g., with CD177) interactions at endothelial junctions, contributing to cell-cell adhesion and neutrophil transmigration.
• Through homophilic ligation, PECAM1 transmits a detachment signal that prevents macrophage-mediated phagocytosis of viable leukocytes, while promoting tethering and phagocytosis of apoptotic leukocytes.
• PECAM1 modulates bradykinin receptor BDKRB2 activation and regulates ERK1/2 signaling in endothelial cells in response to bradykinin and hyperosmotic shock.
• Genetic studies link PECAM1 to the development of atherosclerosis; it induces susceptibility to atherosclerotic lesion formation. Related references: PMID:19048083
• Subcellularly, PECAM1 localizes to the cell membrane, membrane rafts, and cell junctions, consistent with its adhesion and signaling functions.
• In mouse, PECAM1 protein is expressed in lung tissue and platelets, highlighting its vascular and hematopoietic roles.

Experimental Guidance and Technical Tips

• The immunogen (aa 18–220 of mouse CD31) targets the N-terminal extracellular region, which is likely accessible on live cells; consider this when designing flow cytometry panels with other surface markers.
• For flow cytometry, validate the antibody in the relevant sample system under optimized staining conditions (e.g., titration, incubation time, temperature) and include appropriate isotype and viability controls.
• If targeting CD31 in intracellular compartments or under conditions where it may be shed, evaluate the need for fixation/permeabilization protocols while confirming that the epitope remains accessible.
• Given the reported multiple protein weights, confirm the specificity of the conjugate by running a Western blot on reducing/non-reducing gels using a secondary detection reagent if needed.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop sourcing partner for IVD raw materials, CamelBio provides diagnostic manufacturers and research institutions with validated antibodies, bulk ancillary reagents, and customized procurement of rare targets. This anti-CD31/PECAM1 monoclonal antibody supports vascular biology, immunology, and inflammation research, and we can supply matched antibody pairs, controls, and technical consulting to accelerate your assay development from concept to clinic.