Anti-CD317/BST2 Monoclonal Antibody for Flow Cytometry - Q10589

Core Product Specifications and Parameters

Biological Background: BST2 Function and Localization

• BST2 (bone marrow stromal antigen 2), also known as tetherin or HM1.24, is an interferon-induced antiviral host restriction factor encoded by the BST2 gene in humans.
• It efficiently blocks the release of diverse enveloped viruses by tethering nascent virions to the cell membrane, preventing their cell-free spread. This activity has been demonstrated against HIV-1, HIV-2, SIVs, EIAV, FIV, HTLV-1, HCV, Ebola virus, Marburg virus, Lassa virus, SARS-CoV, and many others. Related references: PMID:18200009 PMID:18342597 PMID:19036818 PMID:19179289
• Two isoforms exist due to alternative initiation: isoform 1 and isoform 2. Both function as viral restriction factors but differ in signaling activity and sensitivity to HIV-1 Vpu-mediated degradation. Isoform 2 is resistant to Vpu and restricts HIV-1 budding even in its presence. Related references: PMID:23028328 PMID:26172439
• BST2 localizes to the cell membrane (including lipid rafts), the trans-Golgi network, late endosomes, and the apical cell membrane in polarized epithelial cells.
• It also plays a role in regulating cell growth and migration by inhibiting cell surface proteolytic activity of MMP14, leading to decreased MMP15 activation. Related references: PMID:22065321
• BST2 can stimulate signaling by LILRA4/ILT7 on plasmacytoid dendritic cells, providing negative feedback to IFN production during viral infection. Related references: PMID:19564354 PMID:26172439
• The protein is predominantly expressed in liver, lung, heart, and placenta, with lower levels in pancreas, kidney, skeletal muscle, and brain. It is highly expressed during B-cell development and on T-cells, monocytes, NK cells, and dendritic cells. Overexpression is observed in multiple myeloma cells.
• Key post-translational modifications include a GPI-anchor, disulfide bonds, and N-linked glycosylation. It also features a coiled-coil domain and a transmembrane helix.

Experimental Guidance and Technical Tips

• As an -conjugated monoclonal antibody, this product is suited for direct flow cytometry detection of human CD317/BST2 on viable cells. Ensure proper controls and optimize titration for each experimental system.
• CD317 is a surface and intracellular protein; consider permeabilization protocols for total protein detection.
• Validate reactivity in the desired sample type (e.g., whole blood, PBMCs, cultured cell lines) as expression levels can vary.
• For multicolor panels, verify spectral compatibility with and other fluorophores.

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