Anti-CD317 Rabbit Polyclonal Antibody for WB, ELISA - Q8R2Q8

Core Product Specifications and Parameters

Biological Background: CD317/Bst2 Function and Localization

• CD317, also known as bone marrow stromal antigen 2 (BST-2), HM1.24 antigen, or DAMP-1, is an interferon-inducible host restriction factor that blocks the release of diverse enveloped viruses by tethering nascent virions to the cell membrane.
• It acts as a physical tether, linking virions to the cell surface and to each other, leading to their internalization and degradation or retention, thereby limiting cell-free viral spread.
• Targets include retroviruses (HIV-1, MMTV, MLV), filoviruses (Ebola virus), arenaviruses (Lassa virus), and rhabdoviruses (VSV).
• Beyond antiviral activity, CD317 inhibits cell surface proteolytic activity of MMP14, reducing MMP15 activation and suppressing cell growth and migration.
• It provides negative feedback on IFN production by plasmacytoid dendritic cells through LILRA4/ILT7 signaling.
• Subcellular localization: Golgi apparatus, trans-Golgi network, cell membrane, late endosomes, membrane rafts, and apical cell membrane; found both at the cell surface and intracellular compartments.
• In naive mice, CD317 protein is specifically expressed on type I interferon-producing cells (plasmacytoid dendritic cells).
• Key structural features: single transmembrane domain, coiled-coil region, disulfide bonds, GPI-anchor; it is a glycoprotein and lipoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 55-152 of mouse CD317, covering a portion of the extracellular domain; the antibody may recognize both unglycosylated and glycosylated forms.
• For Western blot, expect a band near 19 kDa, with possible additional bands due to post-translational modifications (e.g., glycosylation) or dimerization.
• Validate the antibody in relevant mouse cell lines or tissues known to express CD317, such as plasmacytoid dendritic cells or interferon-treated cells.
• For ELISA, consider that the polyclonal nature may provide robust signal capture; optimization of coating and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, serving diagnostic manufacturers and research institutions. Our CD317/Bst2 polyclonal antibody supports studies on innate antiviral immunity and viral restriction mechanisms. In addition to this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development.