Anti-CD31 (PECAM1) Monoclonal Antibody for Flow Cytometry - Q3SWT0

Core Product Specifications and Parameters

Biological Background: CD31 Function and Localization

• CD31, also known as PECAM1, is a cell adhesion molecule required for leukocyte transendothelial migration (TEM) under most inflammatory conditions.
• It plays a critical role in endothelial cell-cell adhesion through homophilic interactions and heterophilic binding with CD177 to facilitate neutrophil TEM.
• CD31 homophilic ligation prevents macrophage-mediated phagocytosis of viable leukocytes by transmitting a detachment signal, while promoting phagocytosis of apoptotic leukocytes.
• The protein contains immunoglobulin domains, is heavily glycosylated, and features disulfide bonds and phosphorylation sites.
• It modulates bradykinin receptor BDKRB2 activation and regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells.
• Subcellularly, CD31 localizes to the cell membrane, membrane rafts, and cell junctions.
• It is implicated in susceptibility to atherosclerosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region of rat CD31, ensuring recognition of the native protein on the cell surface.
• As a directly conjugated antibody (ABflo 594), it is optimized for flow cytometry without the need for secondary antibodies.
• For best results in FC, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Validate the antibody in your system using appropriate positive and negative controls (e.g., endothelial cells vs. CD31-negative cells).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated antibodies like this anti-CD31 clone for endothelial and immune cell studies. Our portfolio encompasses optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, enabling streamlined development from concept to clinical application.