Anti-CD31 Monoclonal Antibody () for Flow Cytometry - Q3SWT0

Core Product Specifications and Parameters

Biological Background: CD31 Function and Localization

• CD31 (PECAM1) is a cell adhesion molecule of the immunoglobulin superfamily, critical for leukocyte transendothelial migration (TEM) during inflammation.
• Tyr-660 phosphorylation is essential for PECAM1 trafficking to the lateral border recycling compartment (LBRC) and efficient TEM.
• Mediates homophilic endothelial cell-cell adhesion at junctions and heterophilic interaction with CD177 to facilitate neutrophil TEM.
• Homophilic PECAM1 ligation transmits a detachment signal to prevent phagocytosis of viable leukocytes, while disabled in apoptotic cells, allowing their clearance.
• Modulates bradykinin receptor BDKRB2 activation and regulates hyperosmotic shock-induced ERK1/2 signaling in endothelial cells.
• Localized to the cell membrane, membrane rafts, and cell junctions, consistent with its adhesion and signaling roles.
• Contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and is a glycoprotein with disulfide bonds, classifying it as a phosphoprotein.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein spanning the extracellular domain (aa 18-585) of rat CD31, suggesting it may recognize native PECAM1 on live cells for flow cytometry.
• offers bright far-red fluorescence; titrate the antibody (e.g., 0.5–5 µg/mL) to optimize staining index in your experimental system.
• Use appropriate rabbit IgG isotype control to gate for non-specific binding.
• Validate CD31 expression on rat endothelial cells (e.g., RBE4) or platelets, and include Fc receptor blocking if using samples with high FcγR expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, including this anti-rat CD31 monoclonal antibody () for flow cytometry, supporting cell adhesion and inflammation research. As a trusted partner, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your diagnostic development.