Anti-CD307b Rabbit Monoclonal Antibody for WB, IF/ICC, FC, ELISA - Q96LA5

Core Product Specifications and Parameters

Biological Background: CD307b (FCRL2) Function and Localization

• Fc receptor-like protein 2 (FCRL2), also known as CD307b, is a member of the Fc receptor-like family. It is encoded by the FCRL2 gene in humans, with aliases including FcRH2, IFGP4, IRTA4, and SPAP1.
• The protein may play a regulatory role in both normal and neoplastic B cell development, as indicated by its expression in mature B-cell lineages.
• Its subcellular location is the cell membrane, consistent with its function as a transmembrane receptor.
• Key structural features include multiple immunoglobulin domains, disulfide bonds, and a transmembrane helix; it is also a glycoprotein.
• Tissue expression is predominantly in secondary lymphoid organs such as spleen, lymph node, and tonsil, specifically within B-cell populations, including memory B cells. Multiple isoforms exist due to alternative splicing.
• According to UniProt, it is classified under keywords: receptor, alternative splicing, signal, transmembrane, and glycoprotein, among others.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular region (amino acids 20–395), suggesting the antibody likely targets the extracellular domain of CD307b.
• For Western blotting, anticipate a band around 56 kDa (observed molecular weight) under reducing conditions, taking into account potential disulfide bonds.
• In immunofluorescence/immunocytochemistry, expect membrane staining in B-cell lines; optimize permeabilization for intracellular targets if needed.
• For flow cytometry, we recommend titrating the antibody on CD307b-expressing B-cell lines to determine the optimal concentration.
• ELISA performance can be evaluated using recombinant CD307b protein or native protein from cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with high-quality IVD raw materials, including this anti-CD307b (FCRL2) monoclonal antibody for B-cell development studies. Our extensive catalog features validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents, complemented by rare target sourcing capabilities. We streamline your supply chain from concept to clinic, ensuring reliable access to critical raw materials for assay development.