Anti-CD304/Neuropilin-1 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P97333

Core Product Specifications and Parameters

Biological Background: CD304/Neuropilin-1 Function and Localization

• Neuropilin-1 (also known as A5 protein) is a transmembrane receptor encoded by the Nrp1 gene in mouse, functioning as a co-receptor for semaphorins and members of the vascular endothelial growth factor (VEGF) family.
• It plays essential roles in cardiovascular development, angiogenesis, and neuronal circuit formation, mediating chemorepulsive responses to semaphorin 3A (SEMA3A). Related references: PMID:26503042
• NRP1 recognizes a C-end rule (CendR) motif (R/KXXR/K) on its ligands, promoting cellular internalization and vascular leakage. It binds to VEGF165, VEGFB, and PLGF-2, and co-expression with KDR/VEGFR2 enhances VEGF165 binding and chemotaxis.
• In the nervous system, NRP1 guides motor neuron axon guidance and cell body migration; it is required for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Related references: PMID:26503042
• Beyond its cell surface roles, NRP1 localizes to the mitochondrial membrane and is involved in mitochondrial iron transport via interaction with ABCB8/MITOSUR.
• The protein undergoes post-translational modifications including phosphorylation and glycosylation, and contains disulfide bonds, heparan sulfate attachment, and heparin-binding domains, classified as a proteoglycan.
• Tissue expression is prominent in the nervous system, and it is also found in developing vasculature.

Experimental Guidance and Technical Tips

• For Western blotting, consider using this antibody on mouse tissue lysates; the expected molecular weight is approximately 103 kDa. Optimal antibody dilution should be determined empirically.
• In immunofluorescence/immunocytochemistry (IF/ICC), membrane localization may be observed; fixation and permeabilization conditions should be optimized for the specific cell type.
• For immunohistochemistry of paraffin-embedded tissues (IHC-P), validation on neural tissue sections is recommended, and antigen retrieval methods may be necessary.
• As a recombinant monoclonal antibody, it offers high batch-to-batch consistency and specificity; cross-reactivity with human and rat homologs allows multi-species studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, bulk reagents, and customized supply solutions for diagnostic development. This anti-Neuropilin-1 monoclonal antibody is relevant for angiogenesis and neuroscience research applications. For projects requiring rare target sourcing or technical support from concept to clinic, CamelBio provides comprehensive procurement and consulting capabilities.