Anti-CD300LB Polyclonal Antibody for WB, ELISA - A8K4G0

Core Product Specifications and Parameters

Biological Background: CD300LB Function and Localization

• CD300LB (CD300 molecule like family member B), also known as CMRF35-like molecule 7, CLM-7, IREM-3, and TREM-5, is a single-pass type I membrane protein belonging to the CD300 family of immune receptors.
• The protein acts as an activating receptor, signaling through association with the ITAM-bearing adaptor TYROBP (DAP12) and independently via direct recruitment of GRB2, thereby modulating immune cell activity.
• CD300LB is characterized by a single extracellular immunoglobulin (Ig)-like domain, a transmembrane helix, and a cytoplasmic tail, with structural features including disulfide bonds and phosphorylation sites.
• It is expressed exclusively in myeloid lineage cells, highlighting its role in innate immune responses.
• Subcellularly, CD300LB localizes to the cell membrane, consistent with its function as a surface receptor.
• The human CD300LB gene encodes a protein with a predicted molecular weight of approximately 23 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 1-201) of human CD300LB, encompassing the Ig-like domain; consider this target region when designing detection assays or interpreting cross-reactivity with orthologs.
• For Western blot, consider using lysates from human, mouse, or rat myeloid cells as positive controls, given the myeloid-restricted expression of CD300LB.
• In ELISA applications, recombinant CD300LB protein or cell-based assays may serve as suitable detection systems; validate the antibody’s linear range and limit of detection in your specific matrix.
• As a polyclonal antibody, it may recognize multiple epitopes; verify specificity by including appropriate negative controls, such as CD300LB-deficient cell lines or tissues.
• Shipping on ice bags: upon receipt, aliquot and store at recommended temperatures (typically -20°C or -80°C for long-term storage) to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers comprehensive IVD raw material solutions, equipping diagnostic manufacturers, labs, and research institutes with validated antibodies, bulk ancillary reagents, and rare target sourcing capabilities. This anti-CD300LB polyclonal antibody is part of our immunology portfolio, supporting myeloid cell biology and immune receptor studies. Whether you require catalog-ready reagents or custom development, CamelBio bridges the gap from concept to clinic with reliable, one-stop access.