Anti-CD300C Rabbit Monoclonal Antibody for IF/ICC, FC, ELISA - Q08708

Core Product Specifications and Parameters

Biological Background: CD300C Function and Localization

• CD300C (CMRF35-like molecule 6, also designated CMRF35-A1, CMRF-35, IgSF16) is a single-pass type I transmembrane glycoprotein belonging to the CD300 family of immunoregulatory receptors.
• It is characterized by an extracellular immunoglobulin-like domain, a transmembrane helix, and a cytoplasmic tail, consistent with its classification in the immunoglobulin superfamily.
• Subcellular localization: Cell membrane; the protein is expressed on the cell surface as a receptor.
• Tissue specificity: Present on the surface of monocytes, neutrophils, a proportion of peripheral blood T- and B-lymphocytes, and lymphocytic cell lines.
• Keywords: Receptor, immunity; CD300C is implicated in modulating immune cell activity.
• The precursor protein contains a signal peptide and disulfide bonds, with a predicted mature molecular mass of approximately 24.8 kDa (UniProt); the supplier reports an observed band around 25 kDa under reducing conditions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 21–183) of human CD300C, suggesting the antibody recognizes the native surface receptor; consider using non-permeabilized staining protocols for flow cytometry and immunofluorescence/ICC to detect cell surface expression.
• For intracellular detection, permeabilization may be necessary; validate the protocol in your sample system.
• Suitable sample types include primary human leukocytes (monocytes, neutrophils, T and B cells) and CD300C-expressing cell lines.
• As a monoclonal antibody, it offers lot-to-lot consistency; however, optimal working dilutions must be determined empirically for each assay and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined one-stop platform for sourcing IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. This anti-CD300C rabbit monoclonal antibody supports immunology research and diagnostic development targeting leukocyte surface receptors. For rare targets or custom sourcing needs, our team can assist in securing specialized reagents to bridge the gap from concept to clinic.