Anti-CD300C Polyclonal Antibody for WB, ELISA - Q08708

Core Product Specifications and Parameters

Biological Background: CD300C Function and Localization

• CD300C (CMRF35-like molecule 6) is a member of the CD300 family of immunoregulatory receptors.
• It is a single-pass type I transmembrane glycoprotein containing an extracellular immunoglobulin-like domain.
• The protein is dependent on disulfide bonds for its structure and contains a signal peptide.
• Subcellular localization is at the cell membrane.
• It is expressed on the surface of monocytes, neutrophils, and subsets of peripheral blood T and B lymphocytes.
• The protein is also detected in lymphocytic cell lines.
• Keywords indicate involvement in immunity and receptor signaling.
• The predicted molecular weight is approximately 24.8 kDa, with glycosylation contributing to a higher apparent weight on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 21-183) of human CD300C, suggesting the antibody may recognize the native conformation. Validate specificity in the relevant sample system.
• For WB, consider using whole cell lysates from human monocytes or lymphocytic cell lines as positive controls. Mouse and rat orthologs may also be tested in appropriate cell lines.
• For ELISA, direct coating with recombinant CD300C protein may be applicable; optimize coating conditions and antibody dilution.
• As a polyclonal antibody, lot-to-lot consistency should be monitored, and this antibody may detect additional bands due to cross-reactivity with related family members.

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