Anti-CD300a/c Monoclonal Antibody () for Flow Cytometry - Q9UGN4/Q08708

Core Product Specifications and Parameters

Biological Background: CD300a/c Function and Localization

• CD300A (also known as CMRF35-like molecule 8, IRp60, or IgSF12) is an inhibitory receptor of the CD300 family, with multiple isoforms produced by alternative splicing.
• It down-regulates natural killer (NK) cell cytolytic activity and mast cell degranulation, contributing to immune homeostasis. Related references: PMID:10746781 PMID:16339535 PMID:9701027
• CD300A negatively regulates Toll-like receptor (TLR) signaling mediated by MYD88 through activation of the phosphatase PTPN6, thereby modulating innate immune responses. Related references: PMID:22043923
• The protein localizes to the cell membrane and is widely expressed on immune cells including NK cells, T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes, and monocytes.
• Structurally, it contains an extracellular immunoglobulin domain, a transmembrane helix, and intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs), classifying it as a member of the immunoglobulin superfamily.
• Post-translational modifications include disulfide bond formation, glycosylation, and phosphorylation, which are critical for its signaling function.
• CD300A is implicated in cell adhesion and immune regulatory processes, with potential roles in allergy, autoimmunity, and cancer immunity.

Experimental Guidance and Technical Tips

• This -conjugated monoclonal antibody is designed for flow cytometry (FC) applications. When planning experiments, consider that CD300a/c expression is expected on a variety of human immune cells; titrate the antibody to optimize the staining index in your specific sample system.
• The immunogen corresponds to the extracellular domain (amino acids 18-178) of human CD300a, which may enhance the likelihood of recognizing native cell surface epitopes.
• Given the overlapping expression and homology of CD300 family members, validate specificity using appropriate isotype controls and, if possible, knockout or knockdown cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by offering accessible, high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For studies investigating immune regulation and CD300 family receptors, this CD300a/c antibody is one of many tools available to accelerate your assay development. Contact us to discuss customized bulk requirements or complementary reagents that streamline your workflow from concept to clinic.