Anti-CD300A Rabbit Polyclonal Antibody for WB, ELISA - Q9UGN4

Core Product Specifications and Parameters

Biological Background: CD300A Function and Localization

• Acts as an inhibitory receptor that contributes to the down-regulation of cytolytic activity in natural killer (NK) cells and mast cell degranulation.
  Related references:
  PMID:10746781, PMID:16339535, PMID:9701027
• Negatively regulates Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6.
  Related references:
  PMID:22043923
• Belongs to the CD300 family of immunoglobulin-like receptors; also known as CMRF-35-H9, IRp60, IGSF12, and NK inhibitory receptor.
• Predominantly localized to the cell membrane as a single-pass type I membrane protein.
• Widely expressed in immune cells, including NK cells, T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes, and monocytes.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bonds; a 3D structure has been determined, and alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 18‑180) of human CD300A, making this antibody suitable for detecting the receptor in cell lysates and supernatants.
• For Western blot, consider using reducing conditions; the predicted molecular weight is 33 kDa, but glycosylation may cause band shifts. Validate the observed band against appropriate positive controls.
• For ELISA, plate coating with recombinant CD300A or use of this antibody as a capture/detection component in sandwich ELISAs can be explored; titrate antibody concentrations to optimize signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, CROs, and research institutes with a single access point for high‑quality IVD raw materials and integrated technical services. This anti‑CD300A polyclonal antibody supports studies on immune inhibitory receptors and NK cell function; CamelBio can also supply validated antibody pairs, recombinant proteins, and bulk ancillary reagents to streamline your assay development. Contact us to discuss custom sourcing needs or obtain a complete raw‑material package for your next project.