Anti-CD300A Rabbit Monoclonal Antibody for WB, FC, ELISA - Q9UGN4

Core Product Specifications and Parameters

Biological Background: CD300A Function and Localization

• CD300A functions as an inhibitory receptor that down-regulates cytolytic activity in natural killer (NK) cells and mast cell degranulation. Related references: PMID:10746781 PMID:16339535 PMID:9701027
• It negatively regulates Toll-like receptor (TLR) signaling mediated by MYD88 (but not TRIF) through activation of PTPN6. Related references: PMID:22043923
• Subcellular localization: Cell membrane (single-pass type I membrane protein).
• Tissue specificity: Expressed on NK cells, T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes, and monocytes.
• Belongs to the CD300 family of immunoglobulin-like receptors (IgSF12).
• Post-translational modifications include glycosylation and phosphorylation.
• Alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 18-178), suggesting the antibody targets an epitope within this region. Confirm reactivity in your specific assay.
• For Western blotting, multiple bands may be detected due to alternative splicing—validate band sizes against reported isoforms (33, 20, 12, and 16.5 kDa).
• In flow cytometry, use appropriate single-stain controls and titrate the antibody to optimize signal-to-noise ratio for human leukocytes.
• For ELISA, empirically determine optimal coating/detection antibody concentrations and buffer conditions.
• Cross-reactivity is reported only for human; test other species if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized provider of IVD raw materials, supporting diagnostic developers from concept to clinic. This CD300A antibody addresses needs in immune function research, and we can also supply validated antibody pairs, bulk reagents, and assist with rare target sourcing. Our comprehensive portfolio simplifies your supply chain for immunology-related diagnostic applications.