Anti-CD30 Rabbit Polyclonal Antibody for WB, ELISA - P28908

Core Product Specifications and Parameters

Biological Background: CD30 Function and Localization

• CD30, also known as TNFRSF8 or Ki-1 antigen, is a member of the tumor necrosis factor receptor superfamily and a lymphocyte activation marker.
• It functions as a receptor for CD30L (TNFSF8), which is implicated in the regulation of cellular growth and transformation of activated lymphoblasts. Related references: PMID:8391931
• CD30 signaling can activate the NF-kappa-B pathway, influencing gene expression related to cell survival and proliferation. Related references: PMID:8999898
• The protein is localized to the cell membrane and cytoplasm, consistent with its role as a transmembrane receptor.
• Alternative splicing and alternative initiation generate multiple isoforms of CD30.
• Post-translational modifications include phosphorylation and glycosylation; the protein contains disulfide bonds and a signal peptide.
• CD30 is expressed in activated T and B lymphocytes and detected in alveolar macrophages at the protein level.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 500-595 of human CD30; cross-species reactivity should be verified by sequence alignment if required.
• For Western blotting, consider using human-derived samples such as Hodgkin lymphoma cell lines, and include appropriate loading controls and molecular weight markers around 64 kDa.
• In ELISA applications, optimize coating conditions and validate with CD30L-binding assays to confirm functional epitope recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our CD30 antibody supports studies in lymphoblast activation and lymphoma research. We offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to meet your research and diagnostic needs.