Anti-CD30 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, FC, ELISA - P28908

Core Product Specifications and Parameters

Biological Background: CD30 Function and Localization

• CD30, also known as TNFRSF8 or Ki-1 antigen, is a member of the tumor necrosis factor receptor superfamily. It is encoded by the TNFRSF8 gene (aliases CD30, D1S166E).
• It functions as a receptor for the ligand TNFSF8/CD30L and may regulate cell growth and transformation in activated lymphoblasts. It activates NF-kappa-B to modulate gene expression. Related references: PMID:8391931 PMID:8999898
• Alternative initiation and alternative splicing generate multiple isoforms.
• The protein localizes to the cell membrane and cytoplasm.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation. It contains a signal peptide, transmembrane helix, and repeat regions.
• CD30 is expressed in alveolar macrophages at the protein level.
• It belongs to the TNF receptor family and has a reference proteome status.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 19-379), making this antibody suitable for detecting cell surface CD30 in applications such as flow cytometry, IHC, and live-cell IF/ICC.
• For Western blot, multiple bands around 14 kDa, 50 kDa, and 63 kDa may be observed due to glycosylation and isoform variations. Validate with a positive control lysate from a CD30-expressing cell line (e.g., Hodgkin lymphoma cells).
• Consider performing antigen retrieval (heat-induced epitope retrieval, pH 6 or 9) for IHC-P staining of formalin-fixed paraffin-embedded tissues.
• For IF/ICC, optimize fixation and permeabilization conditions to balance antigen detection and cellular morphology.
• Validate the antibody in the relevant sample system and assay format before routine use, as performance may vary depending on target expression levels and sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for IVD manufacturers and research laboratories, offering one-stop access to high-quality raw materials. This anti-CD30 monoclonal antibody supports lymphoma diagnostic assay development, and we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For custom requirements or rare target sourcing, our technical consulting and supply chain expertise ensure seamless integration from concept to clinic.