Anti-CD3 Rabbit Monoclonal Antibody for Flow Cytometry - P07766

Core Product Specifications and Parameters

Biological Background: CD3 Function and Localization

• CD3E encodes the epsilon chain of the T-cell surface glycoprotein CD3, a core component of the TCR-CD3 complex on T lymphocytes that is essential for adaptive immune response. Related references: PMID:15294938 PMID:15546002 PMID:2470098
• Upon TCR engagement by antigen-presenting cells, the CD3 chains (CD3D, CD3E, CD3G, and CD247/CD3Z) transmit signals across the membrane; their cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) become phosphorylated by Src family kinases LCK and FYN. Related references: PMID:2470098 PMID:40592325
• Phosphorylated ITAMs on CD3E create docking sites for ZAP70, leading to ZAP70 phosphorylation and activation, which triggers downstream signaling cascades required for T-cell activation.
• CD3E plays an essential role in correct T-cell development, ensuring proper thymocyte maturation (By similarity).
• The protein participates in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in its cytosolic region. Related references: PMID:10384095 PMID:26507128
• In addition to its coreceptor function, CD3E serves as a receptor for ITPRIPL1, which inhibits T-cell activation by promoting NCK1 recruitment, thereby disrupting CD3E-ZAP70 interaction and blocking the ERK-NFkB signaling cascade and calcium influx. Related references: PMID:12110186 PMID:38614099
• The CD3 epsilon chain is a single-pass type I membrane protein located at the cell membrane; it features an extracellular immunoglobulin-like domain, a transmembrane helix, and a cytoplasmic tail containing ITAMs. It undergoes phosphorylation and disulfide bonding, and is classified under keywords such as adaptive immunity, receptor, and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen was designed using amino acids 1–126 of human CD3 epsilon, corresponding to the extracellular domain. This suggests the antibody likely binds an epitope accessible on live cells, making it well-suited for flow cytometry.
• For FC applications, titrate the antibody to determine the optimal concentration, and include appropriate isotype controls, live/dead discrimination, and Fc receptor blocking to minimize nonspecific binding.
• Given documented cross-reactivity with cynomolgus and rhesus monkey, the antibody can be considered for non-human primate studies, but users should validate performance in their specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a streamlined one-stop sourcing solution for IVD raw materials. This anti-CD3 antibody exemplifies our portfolio of validated monoclonal and polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents supporting T-cell research and immune monitoring. From target identification to clinical assay development, we offer comprehensive technical consulting and supply services to accelerate your projects from concept to clinic.