Anti-CD2BP2 Polyclonal Antibody for WB and ELISA - O95400

Core Product Specifications and Parameters

Biological Background: CD2BP2 Function and Localization

• CD2BP2 (CD2 antigen cytoplasmic tail-binding protein 2), also known as U5-52K or SNU40, is a conserved component of the U5 small nuclear ribonucleoprotein (snRNP) particle.
• It plays an essential role in pre-mRNA splicing by participating in the assembly of the spliceosome, the macromolecular machinery responsible for intron removal.
• As part of the U5 snRNP, CD2BP2 contributes to the dynamic rearrangements that occur during spliceosome activation and catalysis.
• The protein localizes to both the cytoplasm and the nucleus, consistent with its involvement in snRNP biogenesis and recycling.
• Post-translational modifications of CD2BP2 include acetylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate its function in splicing.
• It is classified under multiple Gene Ontology and UniProt keyword categories including mRNA processing, mRNA splicing, and proteomics identification, underscoring its broad relevance in RNA biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CD2BP2 protein (amino acids 1-341), and the resulting polyclonal antibody is suitable for detecting endogenous CD2BP2 in human cell lysates by Western blot.
• For Western blot applications, consider using whole‑cell extracts from commonly available human cell lines (e.g., HeLa, HEK293) as positive controls, and validate with appropriate loading controls.
• In ELISA, careful optimization of antigen coating and antibody dilution is recommended; consider using recombinant CD2BP2 as a standard for quantitation.
• Due to its role in splicing, CD2BP2 is expected to be ubiquitously expressed; however, expression levels may vary across tissues and experimental conditions, so include appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to high-quality IVD raw materials for splicing and RNA biology targets like CD2BP2. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your assay development from concept to clinic. Whether you require a specific rare-target antibody or custom formulation, CamelBio’s sourcing expertise helps bridge critical gaps in your research and production pipelines.