Anti-CD2AP Rabbit Polyclonal Antibody for WB and ELISA - Q9Y5K6

Core Product Specifications and Parameters

Biological Background: CD2AP Function and Localization

• Acts as an adapter protein between membrane proteins and the actin cytoskeleton. Related references: PMID:10339567
• May facilitate receptor clustering and cytoskeletal polarity at the T-cell/antigen-presenting cell junction.
• Collaborates with CBLC to modulate RET turnover, converting CBLC from inhibitor to promoter of RET degradation.
• Required for cytokinesis, contributing to successful cell division. Related references: PMID:15800069
• Plays a role in epithelial cell junction formation. Related references: PMID:22891260
• May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerulus.
• Localizes to the cytoplasm, cell projections (ruffles), and cell junctions.
• Widely expressed in fetal and adult tissues; contains SH3 domains and undergoes phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 360-639, corresponding to the C-terminal region; consider confirming specificity using knockout or knockdown controls.
• In WB, a predominant band near 71 kDa is expected; validate detection in the relevant cell or tissue lysate.
• For ELISA applications, the polyclonal nature may offer broad epitope coverage; optimize coating and detection conditions for the specific assay format.
• Cross-reactivity with mouse and rat is predicted; verify performance in those species with appropriate positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines access to high-quality IVD raw materials, including validated antibodies for cytoskeleton and cell junction targets like CD2AP. Our supply chain offers monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to support your diagnostic development from concept to clinic.