Anti-CD298/ATP1B3 Rabbit PolymAb® for WB, IF/ICC, IHC-P, ELISA - P54709

Core Product Specifications and Parameters

Biological Background: Sodium/potassium-transporting ATPase subunit beta-3 Function and Localization

• CD298/ATP1B3 is the beta-3 subunit of the sodium/potassium-transporting ATPase (Na+/K+-ATPase), a multi-subunit enzyme that pumps Na+ out and K+ into the cell using ATP hydrolysis.
• As a non-catalytic regulatory subunit, its exact functional role remains unclear, but it is essential for proper enzyme assembly and activity.
• Localizes to the apical and basolateral cell membranes, as well as melanosomes, indicating roles in polarized transport and pigmentation-related processes.
• Undergoes alternative splicing and is a glycoprotein with disulfide bonds, which may modulate its stability and interaction with the catalytic alpha subunit.
• Associated with proteomics identification and reference proteome datasets, confirming widespread tissue expression.
• The computed molecular weight from the UniProt sequence is approximately 31.5 kDa, but the mature protein may undergo glycosylation, leading to a higher apparent weight on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 180–279 of human ATP1B3, a region that may be accessible in both native and denaturing conditions; consider this when selecting applications.
• For western blotting, a band around 22–32 kDa may be observed due to glycosylation; validate the expected size in your specific sample system.
• Given the antibody's reactivity with human, mouse, and rat, it is suitable for cross-species studies; always include appropriate controls.
• For immunofluorescence and immunohistochemistry, optimize fixation and permeabilization conditions to preserve epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-CD298/ATP1B3 rabbit monoclonal antibody supports research into ion transport and membrane biology. In addition to this product, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD development.