Anti-CD276/B7-H3 Polyclonal Antibody for WB, IHC-P, ELISA - Q5ZPR3

Core Product Specifications and Parameters

Biological Background: CD276/B7-H3 Function and Localization

• CD276 antigen, also known as B7-H3, B7H3, B7RP-2, and 4Ig-B7-H3, is a member of the B7 family of immune checkpoint proteins.
• It exists as two isoforms (isoform 1 and isoform 2) that appear redundant in modulating CD4+ T-cell responses; isoform 2 additionally enhances cytotoxic T-cell induction and interferon-gamma production.
• CD276/B7-H3 may regulate T-cell-mediated immune responses and plays a protective role in tumor cells by inhibiting natural killer cell-mediated lysis, serving as a potential marker for neuroblastoma.
• It may be involved in acute and chronic transplant rejection and in regulating lymphocytic activity at mucosal surfaces.
• The protein could play a key role in establishing a suitable immunological environment at the maternal-fetal interface during pregnancy.
• CD276/B7-H3 is a membrane protein (transmembrane) with an extracellular region containing immunoglobulin-like domains, disulfide bonds, and glycosylation sites.
• It is ubiquitously expressed in many tissues but not detectable in peripheral blood lymphocytes or granulocytes; weakly expressed in resting monocytes, and expressed in dendritic cells, extravillous trophoblast cells, and Hofbauer cells of the placenta.
• Keywords from UniProt: Alternative splicing, glycoprotein, phosphoprotein, disulfide bond, transmembrane helix, signal peptide.

Experimental Guidance and Technical Tips

• This antibody was generated against a synthetic peptide spanning amino acids 29-245 of human CD276/B7-H3 (NP_079516.1), covering part of the extracellular domain; it may recognize both isoforms.
• For Western blot, consider testing at a molecular weight of ~57 kDa under reducing conditions in human cell lysates that express CD276/B7-H3 (e.g., certain tumor cell lines or placental tissues).
• In IHC-paraffin applications, validate antigen retrieval methods due to the target’s membrane localization.
• For ELISA, optimize coating and detection conditions to ensure specificity for native or recombinant CD276/B7-H3.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop provider of IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality antibodies like this CD276/B7-H3 polyclonal antibody. Our portfolio includes validated antibody pairs, recombinant proteins, and bulk ancillary reagents to support immune-oncology and transplant research. With technical consultation and sourcing capabilities, CamelBio helps accelerate your assay development from concept to clinical application.