Anti-CD276/B7-H3 Monoclonal Antibody for Flow Cytometry - Q5ZPR3-2

Core Product Specifications and Parameters

Biological Background: CD276/B7-H3 Function and Localization

• CD276, also known as B7-H3 or 4Ig-B7-H3, is a costimulatory molecule belonging to the B7 superfamily.
• The protein is encoded by the CD276/B7H3 gene and alternative splicing generates multiple isoforms, including isoform 2 (UniProt Q5ZPR3-2).
• Isoform 2 has a calculated molecular weight of 33.8 kDa, but post-translational modifications like glycosylation may result in additional bands at 52 kDa and 57 kDa as observed.
• The designation 4Ig-B7-H3 indicates that the protein contains four immunoglobulin-like domains, typical of this subfamily of immunoregulatory receptors.
• As a costimulatory molecule, B7-H3 is believed to modulate T-cell activation and immune responses, though the exact mechanisms remain under investigation.

Experimental Guidance and Technical Tips

• This antibody is conjugated to , a red fluorescent dye suitable for flow cytometry applications; titrate the antibody to determine the optimal dilution for your specific cell type and staining protocol.
• Use appropriate isotype control antibodies and live/dead discrimination to ensure specific staining.
• The immunogen (aa 29-245) corresponds to the extracellular domain of CD276; thus, the antibody likely detects cell surface expression.
• Cross-reactivity with both human and mouse CD276 has been indicated; validate in the relevant species' sample system (e.g., immune cells, tumor cells) to confirm reactivity and specificity.
• For intracellular staining or phospho-specific detection, consider appropriate fixation and permeabilization methods; however, this antibody may primarily recognize extracellular epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CD276/B7-H3 and other costimulatory targets, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to streamline your immuno-oncology and immune checkpoint reagent supply chain.