Anti-CD276 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q5ZPR3-2

Core Product Specifications and Parameters

Biological Background: CD276 Function and Localization

• CD276 antigen is also known as B7-H3, 4Ig-B7-H3, B7RP-2, and costimulatory molecule.
• It functions as a costimulatory molecule potentially involved in T-cell regulation.
• The gene symbol is CD276 (B7H3), encoding multiple isoforms including the targeted isoform 2.
• Isoform 2 (UniProt: Q5ZPR3-2) has a predicted molecular weight of 33791 Da.
• CD276 belongs to the B7 family of immune checkpoint proteins.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 29-245 of the human CD276 canonical sequence, which may confer reactivity to multiple isoforms.
• For Western blotting, a band around 33 kDa is expected under reducing conditions; consider using appropriate positive controls such as cell lines expressing CD276.
• The antibody is suitable for immunohistochemistry (paraffin) and ELISA; optimal dilutions should be determined empirically in the relevant tissue or sample matrix.
• Validate antibody performance in your specific experimental system, as expression levels can vary across cell types and pathological conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of IVD raw materials for diagnostic manufacturers and research laboratories. In addition to this CD276 polyclonal antibody—valuable for immune checkpoint and oncology studies—we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support rare-target sourcing. Our integrated supply chain helps accelerate your projects from concept to clinic.