Anti-CD275 Rabbit Polyclonal Antibody for WB - O75144

Core Product Specifications and Parameters

Biological Background: ICOS Ligand Function and Localization

• ICOS ligand (ICOSL), also known as B7 homolog 2 (B7-H2), B7-like protein Gl50, and B7-related protein 1 (B7RP-1), is a member of the B7 family of immune costimulatory molecules.
• It serves as a ligand for the T-cell-specific receptor ICOS, delivering a costimulatory signal for T-cell proliferation and cytokine secretion (PubMed:11007762, PubMed:11023515). Related references: PMID:11007762 PMID:11023515
• ICOSL also induces B-cell proliferation and differentiation into plasma cells, highlighting its role in humoral immunity.
• In endothelial cells, ICOSL is required for proper neutrophil transmigration in response to chemoattractants such as CXCL8/IL-8 or N-formyl-methionyl peptides (fMLP) (PubMed:30498080). Related references: PMID:30498080
• The protein is localized to the cell membrane, consistent with its function as a transmembrane ligand.
• ICOSL is expressed on peripheral blood B-cells, monocytes, and monocyte-derived dendritic cells; it is also widely distributed in tissues including brain, heart, kidney, liver, lung, and lymphoid organs.
• Structurally, it contains an immunoglobulin domain, a disulfide bond, a signal peptide, a transmembrane helix, and is subject to N-linked glycosylation.
• Alternative splicing results in multiple transcript variants, potentially modulating its expression and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 19-256) of human ICOSL (NP_056074.1), covering the immunoglobulin-like domain. This design favors recognition of the native protein in Western blot.
• For WB, a band around 33 kDa is expected, though post-translational modifications such as glycosylation may shift the apparent molecular weight. Consider deglycosylation assays to confirm specificity.
• Since the antibody cross-reacts with mouse ICOSL, it is suitable for comparative studies between human and mouse samples. Validate performance in the relevant tissue or cell line (e.g., activated B cells, monocytes).
• As a polyclonal antibody, batch-to-batch consistency should be verified, and use of appropriate positive and negative controls is recommended.
• For optimal results, determine the ideal dilution empirically, starting with a range (e.g., 1:500–1:2000) based on the supplier’s recommendation and your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and clinical institutes with comprehensive access to IVD raw materials, including validated antibodies, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-CD275 polyclonal antibody supports investigations into T-cell costimulation and immune regulation, making it a valuable addition to immunology and inflammation research workflows. With end-to-end support from concept to clinic, CamelBio streamlines procurement and technical consultation for your entire project.