Anti-CD272/BTLA Monoclonal Antibody for WB, IF/ICC, FC, ELISA - Q7TSA3

Core Product Specifications and Parameters

Biological Background: CD272/BTLA Function and Localization

• CD272/BTLA (B- and T-lymphocyte attenuator) is an inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling by recruiting the phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. Related references: PMID:12796776, PMID:14652006
• It may interact in cis or in trans with TNFRSF14, with cis interactions maintaining a resting state in naive T cells, while trans interactions can provide survival signals to effector T cells during adaptive immune responses. Related references: PMID:19915044
• CD272/BTLA is a type I transmembrane glycoprotein containing an extracellular immunoglobulin domain and a cytoplasmic tail with immunoreceptor tyrosine-based inhibitory motifs.
• It is primarily expressed on the surface of splenic T and B cells, and also found in lymph nodes, macrophages, NK cells, and dendritic cells, with very low expression in somatic tissues.
• The protein undergoes alternative splicing and post-translational modifications including glycosylation and phosphorylation.
• Its predicted molecular weight is approximately 34.3 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 30-176), ensuring recognition of the native membrane-bound form; consider using non-reducing SDS-PAGE for WB to preserve disulfide bonds if needed.
• For IF/ICC, fixation and permeabilization conditions should be optimized to detect this membrane protein; mild fixation (e.g., 4% paraformaldehyde) is recommended.
• In flow cytometry, this antibody can be used for surface staining of live cells; include appropriate isotype controls.
• For ELISA applications, the antibody can serve as a capture or detection reagent; pair it with a suitable matched antibody pair after validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing a comprehensive range of IVD raw materials to diagnostic manufacturers and research laboratories worldwide. This anti-CD272/BTLA antibody supports immuno-oncology and immune regulation studies, and is part of our extensive catalog of monoclonal and polyclonal antibodies, validated for multiple immunoassay platforms. Looking for antibody pairs, bulk reagents, or rare target sourcing? Contact us to streamline your procurement process.