Anti-CD272/BTLA Monoclonal Antibody for FC - Q7TSA3

Core Product Specifications and Parameters

Biological Background: CD272/BTLA Function and Localization

• B- and T-lymphocyte attenuator (BTLA; CD272) is an inhibitory receptor belonging to the immunoglobulin superfamily. It contains a signal peptide, an extracellular Ig domain, a transmembrane helix, and a cytoplasmic tail with ITIM and ITSM motifs.
• Upon ligand engagement, BTLA recruits the tyrosine phosphatases SHP-1 (PTPN6) and SHP-2 (PTPN11) to negatively regulate antigen receptor signaling in lymphocytes.
  Related references: PMID:12796776, PMID:14652006
• BTLA interacts with TNFRSF14 (herpesvirus entry mediator, HVEM) in both cis (same cell) and trans (between cells) configurations. Cis interactions inhibit trans signaling in naive T cells to maintain a resting state, while trans interactions provide survival signals to effector T cells during adaptive immune responses.
  Related references: PMID:19915044
• BTLA is primarily expressed in lymphoid organs, including splenic T and B cells, lymph nodes, macrophages, NK cells, and dendritic cells, with very weak expression in somatic tissues. A polymorphic tissue distribution is observed across mouse strains.
• The protein undergoes post-translational modifications such as glycosylation and phosphorylation, and features a disulfide bond that stabilizes its immunoglobulin domain structure.
• Alternative splicing generates multiple isoforms of BTLA, which may modulate its surface expression or ligand interactions.
• Because BTLA functions as a co-inhibitory receptor, it is a potential target for cancer immunotherapy and autoimmune disease research.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 30–176), so this antibody likely recognizes the native surface form of mouse CD272/BTLA.
• Optimize staining with a titration series (e.g., 0.1–5 µg/mL) for your specific sample type; include an appropriate isotype control.
• Perform flow cytometry on freshly isolated or properly cryopreserved mouse splenocytes, lymph node cells, or cultured cell lines to maintain surface expression.
• For direct detection, no secondary antibody is required; minimize light exposure during handling to preserve the fluorophore.
• If exploring applications beyond FC, validate thoroughly with positive and negative controls under the chosen conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by offering a curated portfolio of monoclonal antibodies, including validated clones for immune checkpoint targets like CD272/BTLA. We provide ancillary reagents, bulk supply options, and technical consultation to streamline raw material procurement for flow cytometry, immunology, and immunotherapy-related projects. For rare or custom targets, our sourcing team helps accelerate development from concept to clinical application.