Anti-CD271 (p75NTR) Rabbit Polyclonal Antibody for IHC-P and ELISA - Q9Z0W1

Core Product Specifications and Parameters

Biological Background: CD271 Function and Localization

• CD271, also known as the p75 neurotrophin receptor (p75NTR) or TNFRSF16, is a low affinity receptor that binds mature neurotrophins NGF, BDNF, NTF3, and NTF4.
  Related references: PMID:11559852 PMID:1317267
• It forms a high-affinity heterodimer with SORCS2 for pro-neurotrophins (proNGF, proBDNF, proNT3), critically regulating proBDNF-dependent synaptic plasticity, long-term depression (LTD), and long-term potentiation (LTP).
  Related references: PMID:22155786 PMID:24908487 PMID:27457814
• CD271 can mediate both cell survival and apoptosis in neural cells, underscoring its dual role in neurogenesis and programmed cell death.
  Related references: PMID:11559852 PMID:1317267 PMID:24908487
• Beyond the nervous system, CD271 regulates insulin-dependent glucose uptake by promoting GLUT4 translocation to the cell surface in adipocytes and skeletal muscle via RAB31.
  Related references: PMID:22460790
• It is essential for the circadian oscillation of clock genes (BMAL1, PER1, PER2, NR1D1) in the suprachiasmatic nucleus and liver, linking neurotrophin signaling to metabolic rhythms.
  Related references: PMID:23785138
• Subcellularly, CD271 resides on the cell membrane and is also found in the cytoplasm, perikaryon, growth cones, and dendritic spines; it contains a signal peptide, transmembrane domain, and multiple cysteine-rich repeats, and undergoes glycosylation and phosphorylation.
• Tissue expression is prominent in Schwann cells, embryonic brain, hippocampus, and spinal cord, consistent with its roles in neural development and function.
  Related references: PMID:11559852 PMID:22155786 PMID:27457814
• Additionally, CD271 acts as a cell surface receptor for rabies virus glycoprotein G.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 32–254, corresponding to the extracellular domain of mouse CD271; thus, the antibody likely recognizes the N-terminal extracellular region. For IHC-P, consider optimizing antigen retrieval protocols and validate staining in known positive tissues such as brain or spinal cord.
• In ELISA, the antibody can serve as a capture or detection reagent, but optimal working concentrations and potential pairings should be determined empirically using recombinant CD271 or positive cell lysates.
• Despite cross-reactivity with human CD271, re-validation in the intended human sample system is advised because the immunogen is of mouse origin; confirm specificity and sensitivity for human targets.
• As a polyclonal antibody, monitor batch-to-batch consistency, particularly for quantitative assays, by performing lot-specific validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers seamless access to a comprehensive range of IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. For CD271, a key player in neurobiology and apoptosis pathways, our platform supports sourcing needs at every development stage. Contact us to discover how our one-stop service can streamline your assay development from concept to clinic.