Anti-CD27 Monoclonal Antibody for WB, IF-P, ELISA - P26842

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27 is a costimulatory immune-checkpoint receptor belonging to the tumor necrosis factor receptor superfamily (TNFRSF7), expressed on the surface of T-cells, NK-cells, and B-cells. Related references: PMID:28011863
• Upon binding to its ligand CD70, CD27 activates the TRAF2-PTPN6 signaling axis that inhibits LCK phosphorylation, promoting T-cell memory formation and transcriptional adaptation. Related references: PMID:38354704
• The CD70-CD27 pathway is essential for antigen-specific T-cell activation and expansion, which ensures immune surveillance of B-cells. Related references: PMID:28011863
• CD27 provides negative feedback signaling during leukocyte differentiation, modulating hematopoiesis in immune activation.
• In adipose tissue, CD27 negatively regulates the function of Th2 lymphocytes, potentially influencing metabolic inflammation.
• Subcellularly, CD27 localizes to the cell membrane as a single-pass type I transmembrane protein.
• The protein undergoes post-translational modifications, including N-glycosylation and phosphorylation, and contains three intramolecular disulfide bonds that stabilize its extracellular domain.
• UniProt annotates CD27 with keywords such as receptor, signal peptide, transmembrane helix, disulfide bond, glycoprotein, phosphoprotein, apoptosis, and disease variant; it is predominantly found in most T-lymphocytes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human CD27, targeting the N-terminal extracellular domain, which is exposed on the cell surface and involved in ligand binding. This design likely generates antibodies that recognize native, surface-expressed CD27.
• For Western blotting, the predicted molecular weight of CD27 is approximately 29 kDa; however, due to glycosylation, it may migrate as a diffuse band between 30–55 kDa. Validate using a positive control lysate, such as Jurkat or other T-cell lines.
• This antibody has been tested for WB, IF-P, and ELISA with human and rat species. For immunofluorescence (IF-P), permeabilization is required to detect intracellular epitopes or to verify surface staining. Always include appropriate negative controls.
• CD27 can be shed from the cell surface upon activation, generating soluble CD27 (sCD27). If detecting sCD27 by ELISA or other methods, consider using cell culture supernatants or serum samples.
• To ensure optimal performance, store the antibody at -20°C in working aliquots to avoid repeated freeze-thaw cycles. Shipping on ice bags indicates that the antibody is stable under cold transport conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop solution for IVD raw material procurement, including validated monoclonal antibodies like this anti-CD27 antibody, supporting immunology, oncology, and infectious disease assay development. As your strategic sourcing partner, CamelBio ensures access to optimized antibody pairs, bulk reagents, and specialized raw materials tailored to your CD27 detection needs, facilitating a seamless path from research to clinical application.