Anti-CD26/DPP4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P27487

Core Product Specifications and Parameters

Biological Background: CD26/DPP4 Function and Localization

• CD26/DPP4 (Dipeptidyl peptidase 4) is a multifunctional cell surface glycoprotein receptor belonging to the serine protease family, also known as ADABP, ADCP-2, DPP IV, and T-cell activation antigen CD26.
• Functions as a costimulatory molecule essential for T-cell receptor (TCR)-mediated T-cell activation, interacting with adenosine deaminase (ADA), caveolin-1 (CAV1), insulin-like growth factor 2 receptor (IGF2R), and protein tyrosine phosphatase receptor type C (PTPRC). Related references: PMID:10900005 PMID:10951221 PMID:11772392
• Exhibits serine exopeptidase (dipeptidyl peptidase) activity that cleaves N-terminal dipeptides from polypeptides with unsubstituted N-termini and penultimate proline, modulating bioactive peptides including chemokines, growth factors, neuropeptides, and hormones like brain natriuretic peptide 32. Related references: PMID:10570924 PMID:16254193 PMID:10593948
• In complex with fibroblast activation protein (FAP), mediates pericellular proteolysis of the extracellular matrix (ECM), facilitating endothelial cell migration and invasion. Related references: PMID:10593948 PMID:16651416
• Localizes to the plasma membrane, enriched in apical membranes, invadopodia, lamellipodia, membrane rafts, and cell junctions; also found secreted.
• Tissue expression is restricted to lymphatic vessels in skin, small intestine, esophagus, ovary, breast, and prostate, with absence in blood vessels and limited expression in colon; detected in intestinal crypt and villous epithelial cells.
• Acts as a receptor for human coronavirus MERS-CoV-2, mediating viral entry.

Experimental Guidance and Technical Tips

• The immunogen (aa 29-766) covers a large extracellular region predicted to generate antibodies against multiple epitopes, potentially suitable for detecting both native and denatured DPP4 in applications like WB, IHC-P, and ELISA.
• For WB, a specific band around 88-110 kDa (glycosylated form) may be observed; consider using positive control lysates from Jurkat T-cells or Caco-2 intestinal epithelial cells, and validate detection in your sample system.
• In IHC-P, antigen retrieval conditions (e.g., citrate buffer, pH 6.0) may need optimization; recommended positive control tissues include human skin, small intestine, or lymphatic-rich tissues.
• Cross-reactivity with mouse and rat allows potential use in rodent models, but validation in species-relevant tissues is advised.
• The antibody can be applied in ELISA for detection or capture of recombinant or endogenous DPP4; pairing with a compatible detection antibody may require empirical optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with streamlined access to high-quality IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For studies on T-cell activation, viral entry (MERS-CoV), and metabolic regulation involving CD26/DPP4, we support assay development with optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. From concept to clinic, CamelBio bridges your raw material needs with reliable supply solutions.