Anti-CD26/DPP4 Rabbit Monoclonal Antibody for WB and ELISA - P27487

Core Product Specifications and Parameters

Biological Background: CD26/DPP4 Function and Localization

• CD26/DPP4 acts as a costimulatory receptor for T-cell activation, interacting with ADA, CAV1, IGF2R, and PTPRC to modulate immune responses. Related references: PMID:10900005, PMID:10951221, PMID:11772392
• It possesses serine dipeptidyl peptidase IV activity, cleaving N-terminal dipeptides from peptides with penultimate proline, thereby regulating chemokines, growth factors, and hormones. Related references: PMID:10570924, PMID:16254193, PMID:10593948
• In complex with FAP, DPP4 promotes pericellular proteolysis of the extracellular matrix, facilitating endothelial migration and invasion. Related references: PMID:10593948, PMID:16651416
• DPP4 serves as the entry receptor for MERS-CoV-2 coronavirus, implicating it in viral pathogenesis.
• The protein localizes to the cell membrane, especially apical surfaces, invadopodia, lamellipodia, and membrane rafts; a secreted soluble form also exists.
• Expressed specifically in lymphatic endothelium within skin, small intestine, esophagus, ovary, breast, and prostate, but absent from blood vessels; high in intestinal crypts and villi, low in colon.
• It is a glycosylated, disulfide-linked transmembrane protein with a molecular weight of approximately 88 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 667-766) of human DPP4, which is part of the extracellular peptidase domain; this region is well-suited for detecting both membrane-bound and soluble forms.
• For Western blot, a band near 88 kDa is anticipated under reducing conditions; consider using freshly prepared membrane fractions or total cell lysates from human T-cell lines or spleen tissue.
• Cross-reactivity with mouse and rat DPP4 is indicated; validation with species-appropriate positive controls is recommended when applying to non-human samples.
• In ELISA applications, DPP4 can be captured from serum or cell culture supernatants; a matched antibody pair may be considered for sandwich format assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material procurement, offering a streamlined path from discovery to clinical application. This anti-CD26/DPP4 monoclonal antibody supports research in immunology, oncology, and infectious disease; we complement it with validated antibody pairs, bulk quantities, and tailored sourcing for auxiliary reagents to accelerate your diagnostic development. Rely on CamelBio for consistent, high-quality raw materials and dedicated technical support.