Anti-CD262/DR5/TRAILR2 Monoclonal Antibody for FC - O14763

Core Product Specifications and Parameters

Biological Background: CD262/DR5/TRAILR2 Function and Localization

• Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B), also known as death receptor 5 (DR5) or TRAIL receptor 2 (TRAIL-R2), is a receptor for the cytotoxic ligand TNFSF10/TRAIL. Upon ligand binding, it recruits FADD and caspase-8 to form the death-inducing signaling complex (DISC), leading to caspase-8 activation and initiation of apoptosis. It also promotes NF-kB activation and is essential for ER stress-induced apoptosis. Related references: PMID:10549288
• Alternative splicing generates multiple isoforms; the canonical isoform is a single-pass type I membrane protein.
• Widely expressed in adult and fetal tissues, with very high levels in many tumor cell lines (HeLa S3, K-562, HL-60, SW480, A-549, G-361) and high expression in heart, lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach, and intestinal tract; not detectable in brain.
• Subcellular location: cell membrane; the extracellular domain contains TNFR-like cysteine-rich repeats.
• Classified as a glycoprotein with intramolecular disulfide bonds, a signal peptide, a transmembrane helix, and a cytoplasmic death domain.
• Key roles in extrinsic apoptotic signaling and cellular stress responses; implicated in cancer biology due to selective apoptotic induction in tumor cells.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 56–182 of the extracellular domain, suggesting the antibody binds an epitope in this region accessible on live cells for flow cytometry.
• For FC, use directly conjugated antibody; include appropriate isotype control and titrate to optimize signal-to-noise ratio.
• Validate staining in known high-expressing tumor lines (e.g., HeLa, K-562, SW480) as positive controls, and consider blocking with recombinant TRAIL if specificity issues arise.
• Note that the 12, 45, and 47 kDa forms likely represent cleaved or glycosylated variants; molecular weight observed may vary depending on cell type and sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents and rare-target sourcing. For apoptosis and oncology research, our portfolio includes high-quality antibodies against TRAIL receptors like CD262/DR5/TRAILR2, helping you streamline assay development from concept to clinic.