Anti-CD252 (OX40 Ligand) Rabbit Monoclonal Antibody for IF/ICC, FC, ELISA - P43488

Core Product Specifications and Parameters

Biological Background: CD252 (OX40 Ligand) Function and Localization

• Tumor necrosis factor ligand superfamily member 4 (TNFSF4), commonly known as OX40 ligand (OX40L) and CD252, is encoded by the Tnfsf4 gene in mouse.
• Functions as a cytokine that binds to its receptor TNFRSF4 (OX40) to co-stimulate T-cell proliferation and cytokine production.
• Belongs to the tumor necrosis factor (TNF) ligand superfamily, characterized by a conserved C-terminal TNF homology domain responsible for receptor binding.
• Localizes to the plasma membrane as a type II transmembrane protein, with an N-terminal cytoplasmic domain, a single transmembrane helix, and a C-terminal extracellular region.
• Predicted to undergo post-translational modifications including glycosylation and disulfide bond formation, typical of TNF family ligands.
• The mature protein has a calculated molecular weight of approximately 22 kDa, consistent with observed migration in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 51-198 of mouse CD252, spanning the extracellular region and including the TNF homology domain; this design favors recognition of the native membrane-bound form.
• For immunofluorescence applications, expect plasma membrane staining; consider co-staining with membrane markers to confirm localization. Permeabilization may not be required for surface detection.
• In flow cytometry, use fresh or properly fixed cells to preserve surface antigen integrity; titrate the antibody to determine optimal staining concentration.
• For ELISA, the antibody may be evaluated as a capture or detection reagent using recombinant mouse CD252 protein.
• As a rabbit monoclonal antibody, it offers high specificity and consistent lot-to-lot performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD manufacturers and research laboratories, offering a broad portfolio of raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you are developing immunoassays targeting CD252 (OX40 Ligand) for immune checkpoint research or require rare raw materials for T-cell biology, our team is equipped to support your projects from early concept through clinical application.