Anti-CD25 Rabbit Polyclonal Antibody - P01589

Core Product Specifications and Parameters

Biological Background: CD25 Function and Localization

• CD25 (also known as p55, IL-2 receptor alpha chain, TAC antigen) is the alpha subunit of the high-affinity interleukin-2 receptor, encoded by the IL2RA gene.
• The receptor binds interleukin-2 (IL-2) and is critical for the regulation of immune tolerance by modulating the activity of regulatory T cells (TREGs), which suppress the activation and expansion of autoreactive T-cells.
• CD25 is a transmembrane glycoprotein that contains extracellular Sushi (CCP) domains and a short cytoplasmic tail. It belongs to the type I cytokine receptor family.
• It is primarily localized on the plasma membrane of activated T cells, but can also be found on regulatory T cells and other immune cells, where it participates in signal transduction upon IL-2 binding.
• The protein undergoes post-translational modifications including glycosylation and disulfide bond formation, which are important for its structural stability and receptor function.
• Mutations in IL2RA are associated with immunodeficiency 41 (IMD41) and susceptibility to type 1 diabetes mellitus (IDDM10), highlighting its essential role in immune homeostasis.
• CD25 is widely used as a marker for Treg cells in immunological studies and is a target for therapeutic antibodies in autoimmune diseases and transplant rejection.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 101-200, which is part of the extracellular domain; thus, the antibody is expected to recognize the mature, surface-expressed form of CD25.
• For Western blot (WB), the predicted molecular weight is approximately 31 kDa, but glycosylation may cause bands to appear at higher molecular weights. Consider using appropriate positive controls (e.g., activated T-cell lysates).
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), paraffin-embedded sections may require antigen retrieval; test in relevant tissue samples known to express CD25, such as lymphoid tissues.
• In flow cytometry (FC), the antibody may be used to detect membrane-bound CD25 on live cells; ensure appropriate isotype controls and titration.
• Cross-reactivity with mouse and rat has been reported, but signal intensity may vary; validate the antibody in your specific model system.
• For ELISA applications, the polyclonal nature may provide enhanced sensitivity; optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers reliable access to IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. This CD25 rabbit polyclonal antibody supports research in immune regulation and autoimmune disease diagnostics. Leverage our global supply network to accelerate your assay development from concept to clinic.