Anti-CD25 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P01589

Core Product Specifications and Parameters

Biological Background: CD25 Function and Localization

• Interleukin-2 receptor subunit alpha (IL2RA), also known as CD25, TAC antigen, or p55, is the alpha chain of the interleukin-2 receptor.
• The receptor binds interleukin-2 and is crucial for regulating immune tolerance by modulating regulatory T cells (TREGs).
• TREGs suppress the activation and expansion of autoreactive T-cells, thereby maintaining peripheral tolerance.
• It is a type I membrane protein with a signal peptide, extracellular sushi domains, a transmembrane helix, and a short cytoplasmic tail.
• Keywords associated with CD25 include glycoprotein, disulfide bond, immunity, and disease variants (e.g., linked to diabetes mellitus).
• The protein has a calculated molecular weight of approximately 30.8 kDa, observed around 31 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22-213), so the antibody likely recognizes an epitope in that region; consider this when designing blocking or competition experiments.
• For Western blot, expect a band at ~31 kDa under reducing conditions; validate in your specific sample matrix (e.g., cell lines expressing CD25).
• For immunofluorescence/ICC, optimize fixation/permeabilization protocols for membrane proteins; this antibody may detect surface CD25 without permeabilization.
• In ELISA, this mAb may serve as a capture or detection reagent; titrate against recombinant IL2RA or lysates from activated human T cells.
• Given its role in TREG biology, the antibody is a useful tool for studying immune regulation in human samples; however, functional assays (e.g., neutralization) require separate validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete suite of IVD raw materials, including validated monoclonal antibodies like anti-CD25, to support diagnostic development and immunology research. Our one-stop platform streamlines procurement of bulk ancillary reagents, optimized antibody pairs, and rare-target sourcing, ensuring you have the critical tools needed from concept to clinic. Whether advancing immune tolerance studies or building regulatory T-cell assays, our reliable supply chain and technical expertise help keep your projects on track.