Anti-CD248/TEM1 Rabbit Polyclonal Antibody for WB, ELISA - Q9HCU0

Core Product Specifications and Parameters

Biological Background: CD248/TEM1 Function and Localization

• CD248 (endosialin, TEM1) is a transmembrane glycoprotein and member of the C-type lectin domain family, characterized by an EGF-like domain and a calcium-dependent carbohydrate-recognition domain.
• It functions as a cell surface receptor involved in angiogenesis, tissue remodeling, and repair, particularly within the tumor microenvironment.
• CD248 positively modulates PDGF receptor signaling to promote pericyte proliferation, supporting vascular maturation. Related references: PMID:20484976
• It acts as a scaffold for coagulation factor X, enhancing the activation of the TF-factor VIIa complex and contributing to thrombotic events.
• The protein modulates insulin signaling by interacting with the insulin receptor and attenuating its autophosphorylation, and it regulates LPS-induced inflammatory cytokine production in macrophages.
• CD248 negatively regulates T-cell proliferation in a context-dependent manner, while in stromal cells it can enhance proliferation. Related references: PMID:21466550
• Subcellularly, CD248 localizes primarily to the plasma membrane and is strongly expressed on tumor endothelial cells, pericytes, and stromal fibroblasts, with minimal expression in normal quiescent endothelium.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and phosphorylation, contributing to its apparent molecular weight of approximately 81 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal extracellular region (amino acids 18-150) of human CD248, favoring recognition of native and denatured forms in WB.
• For western blotting, a band near 81 kDa is expected; consider the possibility of glycovariants or alternative splicing products that may appear as additional bands.
• A recommended positive control includes human tumor endothelial cell lysates or perivascular cell lines where CD248 is highly expressed.
• In ELISA, the polyclonal nature allows for both capture and detection formats; pair with a matched monoclonal antibody if a sandwich immunoassay is designed.
• Validate reactivity in the target sample system, as expression levels can vary significantly between activated and resting endothelial cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering rapid access to validated antibodies, antigens, and ancillary reagents. This CD248 rabbit polyclonal antibody supports research into tumor angiogenesis and vascular biology, and we can also supply optimized antibody pairs, bulk monoclonal/polyclonal preparations, and custom production for rare targets. Our global network ensures consistent lot-to-lot performance for diagnostic development from concept to clinic.