Anti-CD248 Polyclonal Antibody for WB and ELISA - Q9HCU0

Core Product Specifications and Parameters

Biological Background: CD248 Function and Localization

• Protein identity and isoforms: CD248, also known as endosialin or tumor endothelial marker 1 (TEM1), is a cell surface glycoprotein encoded by the CD248 gene. Alternative splicing produces multiple isoforms.
• Core function in angiogenesis and pericyte biology: CD248 positively modulates PDGF receptor signaling to promote pericyte proliferation, playing a key role in angiogenesis and vascular remodeling. Related references: PMID:20484976
• Coagulation, insulin signaling, and inflammation: Acts as a scaffold for factor X to enhance coagulation activation, modulates insulin receptor autophosphorylation, and regulates LPS-induced proinflammatory cytokine production.
• Immune modulation: Differentially regulates T-cell proliferation depending on the cellular context—inhibitory in T-cells but stimulatory in stromal cells. Related references: PMID:21466550
• Subcellular localization: Single-pass transmembrane protein localized to the plasma membrane.
• Tissue specificity: Strongly expressed in tumor endothelial cells, metastatic liver lesions, pericytes, and stromal fibroblasts; absent or barely detectable in normal endothelial cells. Also found during corpus luteum formation and wound healing.
• Post-translational modifications and domains: Contains EGF-like domains, a C-type lectin domain, and multiple disulfide bonds. Classified as a phosphoprotein and glycoprotein with calcium-binding properties.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 18–150) corresponds to the extracellular region of CD248, which is suitable for generating antibodies that recognize the native surface protein. For Western blot, a band near 81 kDa is expected; however, glycosylation may cause a shift.
• When using this polyclonal antibody, consider validating specificity in your system with appropriate negative controls (e.g., knockout cell lines or tissue) and titrate for optimal dilution in both WB and ELISA. Cross-reactivity with mouse and rat CD248 can facilitate translational studies, but sequence differences should be verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with integrated access to IVD-critical raw materials. For angiogenesis, immune oncology, or vascular biology programs, we provide validated antibody pairs, optimized polyclonal and monoclonal reagents, and bulk ancillary supplies—including specific binders to emerging targets like CD248. Our streamlined sourcing model helps you secure rare or custom reagents efficiently, accelerating the transition from concept to clinic.