Anti-CD244 Rabbit Polyclonal Antibody for WB, ELISA - Q9BZW8

Core Product Specifications and Parameters

Biological Background: CD244 Function and Localization

• CD244 is a heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family, with CD48 as its ligand. It modulates immune cell activation and differentiation through homo- or heterotypic cell-cell interactions, linking innate and adaptive immunity.
• CD244 acts as an activating natural killer (NK) cell receptor, stimulating NK cell cytotoxicity, IFN-gamma production, and granule exocytosis. Related references: PMID:10359122, PMID:11714776, PMID:8376943
• Its activating function implicates association with SH2D1A/SAP and FYN, initiating downstream signaling through VAV1, INPP5D/SHIP1, and CBL. Signal attenuation in the absence of SH2D1A involves INPP5D and, to a lesser extent, phosphatases SHP-1 and SHP-2. Related references: PMID:10934222, PMID:15713798
• At early stages of NK cell differentiation, CD244 may function as an inhibitory receptor, potentially ensuring self-tolerance of developing NK cells. Related references: PMID:11917118
• CD244 acts as a costimulatory molecule in NK cells, enhancing signals from other activating receptors such as NCR3 and NCR1. Related references: PMID:10741393
• It is expressed in spleen, peripheral blood leukocytes, lung, liver, testis, and small intestine, and is found on all NK cells, monocytes, basophils, TCR-gamma/delta+ T-cells, and a subset of CD8+ T-cells.
• The protein is a single-pass type I transmembrane receptor located in the cell membrane and membrane rafts. It undergoes alternative splicing and post-translational modifications including glycosylation, disulfide bond formation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (amino acids 22–224), which contains the CD48-binding region; this region may be suitable for detecting CD244 in its native conformation.
• For Western blot, anticipate a band around 42 kDa; post-translational modifications (glycosylation) may cause a shift. Validate using a relevant positive control, such as NK cell lysates.
• ELISA can be used to detect CD244 in biological samples; consider optimizing coating conditions and include appropriate negative controls to ensure specificity.
• Cross-reactivity with mouse CD244 indicates potential use in murine models; however, confirm epitope conservation between species and validate in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with a streamlined path from concept to clinic, offering comprehensive IVD raw materials, technical services, and consulting. This anti-CD244 polyclonal antibody is part of our expanding portfolio for immunological research and NK cell biology, supporting the development of novel diagnostic assays. We also offer customized sourcing for validated antibody pairs, bulk ancillary reagents, and rare target raw materials to accelerate your assay development projects.